The comparison of the effect of acute moderate and high-intensity exercise on the uncoupling protein -1 secretion

Merawati, D., Sugiharto, S., Pranoto, A., Andiana, O., & Angga, P. D. (2022). The comparison of the effect of acute moderate and high-intensity exercise on the uncoupling protein -1 secretion. Jurnal SPORTIF : Jurnal Penelitian Pembelajaran, 8(2), 201 - 216. https://doi.org/10.29407/js_unpgri.v8i2.17674

Abstract

Physical activities accelerate the secretion of the uncoupling protein-1 (UCP-1), which reduces the risk of metabolic disorders and the prevalence of obesity. This research aimed to investigate the effects of acute physical activities on UCP-1 secretion. A total of 20 males (aged between 19-20 years old) were divided into groups with either a 30-minute moderate-intensity exercise (MIE) or high-intensity exercise (HIE). The UCP-1 expression was measured using Enzyme-Linked Immunosorbent Assay (ELISA) (pre and 5 minutes post the exercise). The pre and post-UCP-1 data in each group were compared with a paired t-test, while pre and post UCP data between groups were analyzed using the Independent-Sample T-test. The pre and post test UCP-1 in the MIE was (4.16±0.89) ng/mL and (4.55±1.36) ng/mL (P>0.05), while the pre and post test UCP-1 in the HIE were (4.09±0.53) ng/mL and (5.06±1.02) (P<0.05). No significant difference in pretest UCP-1, however was found between groups (P>0.05) and posted UCP-1 (P>0.05). In conclusion, 30 minutes of high-intensity exercise is required to increase UCP-1 in young adults significantly.

https://doi.org/10.29407/js_unpgri.v8i2.17674

PDF

Similarity Check

References

Adji, B.S., Sugiharto, Merawati, D., & Pranoto, A. (2021). The increase of uncoupling protein-1 expression after moderate intensity continuous exercises in obese females. Jurnal SPORTIF : Jurnal Penelitian Pembelajaran, 7(2), 194-205. https://doi.org/10.29407/js_unpgri.v7i2.15932.

Aldiss P, Betts J, Sale C, Pope M, Budge H, Symonds ME. (2018). Exercise-induced ‘browning’ of adipose tissues. Metabolism. 81: 63-70. https://doi.org/10.1016/j.metabol.2017.11.009.

Andarianto, A., Rejeki, P.S., Sakina, Pranoto, A., Seputra, T.W.A., Sugiharto, & Miftahussurur, M. (2022). Inflammatory markers in response to interval and continuous exercise in obese women. Comparative Exercise Physiology, 18(2), 135 – 142. https://doi.org/10.3920/CEP210038.

Appari M, Channon KM, McNeill E. (2018). Metabolic Regulation of Adipose Tissue Macrophage Function in Obesity and Diabetes. Antioxidants & Redox Signaling. 29: 297-312. https://doi.org/10.1089/ars.2017.7060.

Befroy DE, Petersen KF, Dufour S, Mason GF, Rothman DL, Shulman GI. (2008). Increased Substrate Oxidation and Mitochondrial Uncoupling in Skeletal Muscle of Endurance-Trained Individuals. Proceedings of the National Academy of Sciences of the United States of America. 105: 16701-16706.

Brondani LA, Assmann TS, Duarte GCK, Gross JL, Canani LH, Crispim D. (2012). The role of the uncoupling protein 1 (UCP1) on the development of obesity and type 2 diabetes mellitus. Arquivos Brasileiros de Endocrinologia & Metabologia. 56: 215-225. https://doi.org/10.1590/S0004-27302012000400001.

de Queiroz KB, Rodovalho GV, Guimarães JB, de Lima DC, Coimbra CC. (2021). Evangelista EA, Guerra-Sá R. Endurance training blocks uncoupling protein 1 up-regulation in brown adipose tissue while increasing uncoupling protein 3 in the muscle tissue of rats fed with a high-sugar diet. Nutrition Research. 32: 709-717. https://doi.org/10.1016/j.nutres.2012.06.020.

Daskalopoulou SS, Cooke AB, Gomez Y-H, Mutter AF, Filippaios A, Mesfum ET, Mantzoros CS. (2014). Plasma irisin levels progressively increase in response to increasing exercise workloads in young, healthy, active subjects. European Journal of Endocrinology. 171(3): 343-352. https://doi.org/10.1530/EJE-14-0204.

Dewal RS and Stanford KI. (2019). Effects of exercise on brown and beige adipocytes. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1864(1): 71-78. https://doi.org/10.1016/j.bbalip.2018.04.013.

Dinas PC, Lahart IM, Timmons JA, Svensson PA, Koutedakis Y, Flouris AD, Metsios GS. (2017). Effects of physical activity on the link between PGC-1a and FNDC5 in muscle, circulating Ιrisin and UCP1 of white adipocytes in humans: A systematic review. F1000Research. 6: 286. https://doi.org/10.12688/f1000research.11107.1.

Fenzl, A. and Kiefer, F.W. (2014). Brown adipose tissue and thermogenesis. Horm Mol Biol Clin Invest. 19(1): 25–37. https://doi.org/10.1515/hmbci-2014-0022.

Flouris AD, Dinas PC, Valente A, Andrade CMB, Kawashita NH, Sakellariou P. (2017). Exercise-induced effects on UCP1 expression in classical brown adipose tissue: a systematic review. Hormone Molecular Biology and Clinical Investigation. 31(2): 20160048. https://doi.org/10.1515/hmbci-2016-0048.

Fuller-Jackson J-P and Henry BA. (2018). Adipose and skeletal muscle thermogenesis: studies from large animals. Journal of Endocrinology. 237: R99-R115. https://doi.org/10.1530/JOE-18-0090.

Garneau L, Parsons SA, Smith SR, Mulvihill EE, Sparks LM, Aguer C. (2020). Plasma Myokine Concentrations After Acute Exercise in Non-obese and Obese Sedentary Women. Frontiers in Physiology. 11: 18. https://doi.org/10.3389/fphys.2020.00018.

Gorski T, Mathes S, Krützfeldt J. (2018). Uncoupling protein 1 expression in adipocytes derived from skeletal muscle fibro/adipogenic progenitors is under genetic and hormonal control: UCP1 expression in skeletal muscle FAPs. Journal of Cachexia, Sarcopenia and Muscle. 9: 384-399. https://doi.org/10.1002/jcsm.12277.

Herningtyas EH and Ng TS. (2019). Prevalence and distribution of metabolic syndrome and its components among provinces and ethnic groups in Indonesia. BMC Public Health. 19 (377). https://doi.org/10.1186/s12889-019-6711-7.

Huh JY, Mougios V, Kabasakalis A, Fatouros I, Siopi A, Douroudos II, et al. (2014). Exercise-Induced Irisin Secretion Is Independent of Age or Fitness Level and Increased Irisin May Directly Modulate Muscle Metabolism Through AMPK Activation. The Journal of Clinical Endocrinology & Metabolism. 99(11): E2154–E2161. https://doi.org/10.1210/jc.2014-1437.

Huh J.Y. (2018). The role of exercise-induced myokines in regulating metabolism. Archives of Pharmacal Research. 41: 14-29. https://doi.org/10.1007/s12272-017-0994-y.

Jabbour G and Iancu H-D. (2017). High-intensity exercise training does not influence body weight but improves lipid oxidation in obese adults: a 6-week RCT. BMJ Open Sport & Exercise Medicine. 3(1): e000283. https://doi.org/10.1136/bmjsem-2017-000283.

Khalafi M, Mohebbi H, Symonds ME, Karimi P, Akbari A, Tabari E, Faridnia M, Moghaddami K. (2020). The Impact of Moderate-Intensity Continuous or High-Intensity Interval Training on Adipogenesis and Browning of Subcutaneous Adipose Tissue in Obese Male Rats. Nutrients.12: 925. https://doi.org/10.3390/nu12040925.

Kim N, Kim J, Yoo C, Lim K, Akimoto T, Park J. (2018). Effect of acute mid-intensity treadmill exercise on the androgen hormone level and uncoupling protein-1 expression in brown fat tissue of mouse. Journal of Exercise Nutrition & Biochemistry. 22: 15-21. https://doi.org/10.20463/jenb.2018.0003.

Kim SH and Plutzky J. (2016). Brown Fat and Browning for the Treatment of Obesity and Related Metabolic Disorders. Diabetes & Metabolism Journal. 40(1): 12-21. https://doi.org/10.4093/dmj.2016.40.1.12.

Leal LG, Lopes MA, Batista ML. (2018). Physical Exercise-Induced Myokines and Muscle-Adipose Tissue Crosstalk: A Review of Current Knowledge and the Implications for Health and Metabolic Diseases. Frontiers in Physiology. 9: 1307. https://doi.org/10.3389/fphys.2018.01307.

Mai S, Grugni G, Mele C, Vietti R, Vigna L, Sartorio A, Aimaretti G, Scacchi M, and Marzullo P. (2020). irisin levels in genetic and essential obesity: clues for a potential dual role. Scientific Reports. 10(1020): 1-9. https://doi.org/10.1038/s41598-020-57855-5.

Moienneia N. and Hosseini S.R.A. (2016). Acute and chronic responses of metabolic myokine to different intensities of exercise in sedentary young women. Obesity Medicine. 1: 15-20. http://dx.doi.org/10.1016/j.obmed.2015.12.002.

Morrison S.F. (2016). Central control of body temperature. F1000Research. 5: F1000 Faculty Rev-880. https://doi.org/10.12688/f1000research.7958.1.

Müller MJ, Enderle J, Bosy-Westphal A. (2016). Changes in Energy Expenditure with Weight Gain and Weight Loss in Humans. Current Obesity Reports. 5: 413-423. https://doi.org/10.1007/s13679-016-0237-4.

Oh K-J, Lee D, Kim W, Han B, Lee S, Bae K-H. (2017). Metabolic Adaptation in Obesity and Type II Diabetes: Myokines, Adipokines and Hepatokines. International Journal of Molecular Sciences. 18(1): 8. https://doi.org/10.3390/ijms18010008.

Paradisis GP, Zacharogiannis E, Mandila D, Smirtiotou A, Argeitaki P, Cooke CB. (2014). Multi-Stage 20-m Shuttle Run Fitness Test, Maximal Oxygen Uptake and Velocity at Maximal Oxygen Uptake. Journal of Human Kinetics.41: 81-87. https://doi.org/10.2478/hukin-2014-0035.

Purdom T, Kravitz L, Dokladny K, Mermier C. (2018). Understanding the factors that effect maximal fat oxidation. J Int Soc Sports Nutr.15 (3). https://doi.org/10.1186/s12970-018-0207-1.

Qiu S, Bosnyák E, Treff G, Steinacker JM, Nieß AM, Krüger K, Mooren FC, Zügel M, Schumann U. (2018). Acute exercise-induced irisin release in healthy adults: Associations with training status and exercise mode. European Journal of Sport Science.18: 1226-1233. https://doi.org/10.1080/17461391.2018.1478452.

Reisi, J., Ghaedi, K., Rajabi, H., & Marandi, S. M. (2016). Can Resistance Exercise Alter Irisin Levels and Expression Profiles of FNDC5 and UCP1 in Rats?. Asian journal of sports medicine. 7(4): e35205. https://doi.org/10.5812/asjsm.35205.

Rejeki, P. S., Baskara, P. G., Herawati, L., Pranoto, A., Setiawan, H. K., Lesmana, R., & Halim, S. (2022). Moderate-intensity exercise decreases the circulating level of betatrophin and its correlation among markers of obesity in women. Journal of basic and clinical physiology and pharmacology, 10.1515/jbcpp-2021-0393. Advance online publication. https://doi.org/10.1515/jbcpp-2021-0393.

Rejeki, P.S., Pranoto, A., Prasetya, R.E., & Sugiharto. (2021). Irisin serum increasing pattern is higher at moderate-intensity continuous exercise than at moderate-intensity interval exercise in obese females. Comparative Exercise Physiology, 17(5): 475–484. https://doi.org/10.3920/CEP200050.

Rodrigues KCC, Pereira RM, Campos TDP, Moura RF, Silva ASR, Cintra DE, Ropelle ER, Pauli JR, Araújo MB, Moura LP. (2018). The Role of Physical Exercise to Improve the Browning of White Adipose Tissue via POMC Neurons. Frontiers in Cellular Neuroscience.12 (8). https://doi.org/10.3389/fncel.2018.00088.

Sanchez-Delgado G, Martinez-Tellez B, Olza J, Aguilera CM, Gil Á, Ruiz JR. (2015). Role of Exercise in the Activation of Brown Adipose Tissue. Ann Nutr Metab. 67(1): 21-32. https://doi.org/10.1159/000437173.

Shirvani, H. and Arabzadeh, E. (2020). Metabolic cross-talk between skeletal muscle and adipose tissue in high-intensity interval training vs. moderate-intensity continuous training by regulation of PGC-1α. Eating and Weight Disorders - Studies on Anorexia, Bulimia and Obesity. 25:17–24. https://doi.org/10.1007/s40519-018-0491-4.

Sugiharto, Merawati, D., Pranoto, A., Rejeki, P.S., Lupita, M.N., Adji, B.S., Susanto, H., & Taufiq, A. (2021). Acute Interval and Continuous Moderate-Intensity Exercise Enhanced Circadian Thermogenic Activity through Browning-related Genes in Obese Adolescent Female. Malaysian Journal of Fundamental and Applied Sciences, 17(5), 566-581. https://doi.org/10.11113/mjfas.v17n5.2271.

Sugiharto, S., Merawati, D., Susanto, H., Pranoto, A., & Taufiq, A. (2022). The exercise-instrumental music program and irisin levels in younger non-professional athletes. Comparative Exercise Physiology, 18(1), 65–73. https://doi.org/10.3920/CEP210015.

Sugiharto, Susanto, H, Andiana O, Merawati D. (2019a). Caloric Regulation Linked Thermogenesis in Acute Submaximal Intensity Exercise Model as The Effect of Audio Frequency Exposure. IOP Conference Series: Materials Science and Engineering. 515: 012069. https://doi.org/10.1088/1757-899X/515/1/012069.

Sugiharto, Susanto H, Merawati D, Andiana O. (2019b). The Effect of Tempo of Musical Treatment and Acute Exercise on Vascular Tension and Cardiovascular Performance: A Case Study on Trained Non-Athletes. IOP Conference Series: Materials Science and Engineering. 515: 012033. https://doi.org/10.1088/1757-899X/515/1/012033.

Tsuchiya Y, Ando D, Goto K, Kiuchi M, Yamakita M, Koyama K. (2014). High-Intensity Exercise Causes Greater Irisin Response Compared with Low-Intensity Exercise under Similar Energy Consumption. The Tohoku Journal of Experimental Medicine. 233(2): 135-140. https://doi.org/10.1620/tjem.233.135.

Tsuchiya Y, Ando D, Takamatsu K, Goto K. (2015). Resistance exercise induces a greater irisin response than endurance exercise. Metabolism: Clinical and Experimental. 64(9): 1042-1050. https://doi.org/10.1016/j.metabol.2015.05.010.

Zhang J, Valverde P, Zhu X, Murray D, Wu Y, Yu L, Jiang H, Dard MM, Huang J, Xu Z, Tu Q, Chen J. (2017). Exercise-induced irisin in bone and systemic irisin administration reveal new regulatory mechanisms of bone metabolism. Bone Research. 5: 1-14. https://doi.org/10.1038/boneres.2016.56.