Kata Kunci
intensitas tinggi
latihan
UCP-1
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Abstrak
Aktivitas fisik mempercepat sekresi protein-1 yang tidak terpisahkan (UCP-1), yang mengurangi risiko gangguan metabolisme dan prevalensi obesitas. Penelitian ini bertujuan untuk menyelidiki efek dari aktivitas fisik akut pada sekresi UCP-1. Sebanyak 20 laki-laki (berusia antara 19-20 tahun) dibagi menjadi beberapa kelompok dengan latihan intensitas sedang (MIE) 30 menit atau latihan intensitas tinggi (HIE). Ekspresi UCP-1 diukur menggunakan Enzyme-Linked Immunosorbent Assay (ELISA) (pra dan 5 menit setelah latihan). Data pra dan pasca-UCP-1 di setiap grup dibandingkan dengan uji-t berpasangan, sedangkan data pra dan pasca UCP antar kelompok dianalisis menggunakan uji-T Sampel Independen. Uji pra dan pasca UCP-1 di MIE adalah (4.16±0.89) ng/mL dan (4.55±1.36) ng/mL (P>0.05), sedangkan pre and post test UCP-1 di HIE adalah (4.09±0.53) ng/mL dan (5.06±1.02) (P<0.05). Tidak ada perbedaan yang signifikan dalam UCP-1 pretest, namun ditemukan antara kelompok (P>0.05) dan UCP-1 yang diposting (P>0.05). Kesimpulannya, 30 menit latihan intensitas tinggi diperlukan untuk meningkatkan UCP-1 pada orang dewasa muda secara signifikan.
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